Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (10)
Sample
Human Purified protein (head and neck cancer (AMC HN9))
Specification
head and neck cancer (AMC HN9)
Treatment
0 5 15um DCA for 24h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/2000
Secondary antibody
Name
Abcam antibody:Donkey anti-Rabbit IgG H&L (DyLight 488) secondary antibody
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 46 kDa
Exposure
2 hour(s), 0 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 02 2014