Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (Brain sections)
Permeabilization
Yes - 0.3% TritonX in 0.1% PBS
Specification
Brain sections
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% TritonX with 0.1x PBS- 10% Donkeys
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Alexa Fluro 488
Host species: Pig
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Pig
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
Adult mouse was perfused with 4% PFA in 0.1M PBS. IFC staining performed in brain sections with anti-PDGFß1 (ab32570) with Alexa488. The antibody stained pericytes , endothelial membrane also some cells in blood vessels.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ruma Raha-Chowdhury
Verified customer
提交于 Feb 18 2013