Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Fibroblasts)
Gel Running Conditions
Reduced Denaturing (7,5)
Loading amount
150 µg
Treatment
PDGF 40ng/ml 5min
Specification
Mouse Fibroblasts
Blocking step
PhosphoBlocker as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: RT
Dilution
1/400
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: A-2074
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
untreated NIH3T3 cells
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 180 kDa
Positive control
NIH3T3 cells treated with 40ng/ml PDGF for 5 min
Additional data
Additional Notes
It seems that the Antibody does not detect human PDGFR-alpha.
There is no signal for the U343 and D54 celllysate. (see Picture)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Oct 29 2010