Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Skeletal Muscle (Gastrocnemius))
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris (pH8) better than Citrate (pH6)
Permeabilization
No
Specification
Skeletal Muscle (Gastrocnemius)
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methacarn
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent Reagent
Dilution
1/250
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat Anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
GREEN = PDGF-BB; RED = WGA Membranes; BLUE = DAPI Nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 21 2013