Application
Western blot
Sample
Rat Tissue lysate - whole (Penile Tissue)
Gel Running Conditions
Reduced Denaturing (7.5 % mini gel)
Loading amount
60 µg
Specification
Penile Tissue
Blocking step
Starting Block T20 (PBS) as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
22 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Starting Block T20 (PBS)
Dilution
1/1000
Secondary antibody
Dilution
1/7000
Name
Non-Abcam antibody was used: ECL Anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Chemiluminescence, ECL
Exposure
2 minute(s) and 0 second(s)
Positive control
I used mouse lung as positive control
Additional data
Additional Notes
I ran mouse tissue on the same gel and it worked in mouse tissue but not very well in rat tissue. It may be that when using this particular rat tissue I need to load more protein or make the primary antibdy more concentrated.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Gwen Lagoda
Verified customer
提交于 Jul 14 2009