Application
Western blot
Sample
Human Cell lysate - whole cell (platelet)
Gel Running Conditions
Reduced Denaturing (7.5)
Loading amount
15 µg
Specification
platelet
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS-T
Dilution
1/500
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: Invitrogen A11034
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey
Exposure
1 second(s)
Bands
Specific: 100 kDa Non-specific: 50 kDa
Additional data
Additional Notes
Human platelet lysate was loaded onto a 7.5 % SDS-polyacrylamide gel, transferred to a nitrocellulose membrane and probed with Abcam Antibody, cat. No. Ab64179, against PDE5A. We observed a specific band of approximately 100 kDa MW.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 29 2009