Anti-PDE4D抗体(ab14613)
Key features and details
- Rabbit polyclonal to PDE4D
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-PDE4D抗体
参阅全部 PDE4D 一抗 -
描述
兔多克隆抗体to PDE4D -
宿主
Rabbit -
特异性
ab14613 labels all PDE4D proteins including PDE4D1, PDE4D2, PDE4D3, PDE4D4, PDE4D5 variants. Does not cross react with PDE4A, PDE4B, PDE4D6, PDE4D7, PDE4D8, PDE4D9 or PDE4C. The antibody has not been tested against endogenous PDE4D, only recombinant proteins and we therefore cannot guarantee it will detect endogenous PDE4. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to PDE4D. C-terminal cyclic peptide unique to all PDE4D subtypes variants.
(Peptide available asab169991) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.02% Sodium azide -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab14613于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB | (1) |
1/500.
|
IHC-P |
Use at an assay dependent concentration.
|
说明 |
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WB
1/500. |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. -
组织特异性
Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle. -
通路
Purine metabolism; 3',5'-cyclic AMP degradation; AMP from 3',5'-cyclic AMP: step 1/1. -
疾病相关
Note=Genetic variations in PDE4D might be associated with susceptibility to stroke. PubMed:17006457 states that association with stroke has to be considered with caution. -
序列相似性
Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily. -
翻译后修饰
Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59. -
细胞定位
Cytoplasm. Membrane. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome. - Information by UniProt
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数据库链接
- Entrez Gene: 5144 Human
- Omim: 600129 Human
- SwissProt: Q08499 Human
- Unigene: 117545 Human
- Unigene: 721404 Human
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别名
- cAMP specific 3',5' cyclic phosphodiesterase 4D antibody
- DKFZp686M11213 antibody
- 5''-cyclic phosphodiesterase 4D antibody
see all
图片
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Western blot using ab14613 and positive controls for PDE4D variants.
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ab14613 (2µg/ml) staining PDE4D in human placenta using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of the cytoplasm.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
实验方案
数据表及文件
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Datasheet download
文献 (18)
ab14613 被引用在 18 文献中.
- Huang M et al. Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D. Mol Metab 47:101182 (2021). PubMed: 33549845
- Eyster CA et al. Diabetes induced decreases in PKA signaling in cardiomyocytes: The role of insulin. PLoS One 15:e0231806 (2020). PubMed: 32817622
- Zhou T et al. LncRNA XIST regulates myocardial infarction by targeting miR-130a-3p. J Cell Physiol 234:8659-8667 (2019). PubMed: 29226319
- Civciristov S et al. Preassembled GPCR signaling complexes mediate distinct cellular responses to ultralow ligand concentrations. Sci Signal 11:N/A (2018). PubMed: 30301787
- Pinto CS et al. Phosphodiesterase Isoform Regulation of Cell Proliferation and Fluid Secretion in Autosomal Dominant Polycystic Kidney Disease. J Am Soc Nephrol 27:1124-34 (2016). PubMed: 26289612