Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293T)
Loading amount
10 µg
Specification
HEK 293T
Treatment
Negative Control or PDCD5 siRNA for 96 hrs
Gel Running Conditions
Reduced Denaturing (16.5% acrylamide, tricine)
Blocking step
LiCor Blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1:1 LiCor blocking buffer, PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Dilution
1/10000
Detection
Detection method
Odyssey Infrared Imager
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 14 kDa Non-specific: 30, 32, 50 kDa
Positive control
10 ug of HEK293T cell lysate treated with 100nM non-specific siRNA for 96 hrs
Negative control
10 ug of HEK293T cell lysate treated with 100nM PDCD5 siRNA for 96 hrs
Additional data
Additional Notes
Lane 1 is a Prestained Protein Molecular Weight Marker (Fermentas), 20, 26, 36, 47, 85, 118 kDa.
Lane 2 was treated with 100 nM non-specific siRNA
Lane 3 was treated with 100 nM PDCD5 siRNA
Lane 2 was treated with 100 nM non-specific siRNA
Lane 3 was treated with 100 nM PDCD5 siRNA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 31 2011