Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-12%)
Loading amount
15 µg
Specification
MDCK
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Detection
Detection method
Clarity western ECL substrate
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 32 kDa Non-specific: 48 kDa
Additional data
Additional Notes
ABTRIAL-EVTN3
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 25 2016