Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
2% normal goat serum, 1% bovine serum albumin, 0.1% cold fish skin gelatine, 0.1% Triton X-100, 0.05% Tween 20, 0.05% sodium azide, 0.01M PBS, pH 7.2 as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer (pH 9.0)
Sample
Frog Tissue sections (Proximal Small intestine)
Specification
Proximal Small intestine
Permeabilization
No
Fixative
Formaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1%BSA, 0.1% CFSG, 0.01M PBS
Dilution
1/5000
Additional data
Additional Notes
Worked beautifully! Counterstained with DAPI (Blue). Dual labelled cells = aqua. Species: Green striped burrowing frog (Cyclorana alboguttata).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Rebecca Cramp
Verified customer
提交于 Nov 22 2013