概述

  • 产品名称
  • 描述
    山羊多克隆抗体to PCK2
  • 特异性
    This antibody is expected to recognise isoform 1 (NP_004554) only, no cross-reactivity with PCK1.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Guinea pig, Human
    预测可用于: Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    KPWKPGDKEPCAH

    , corresponding to Internal sequence amino acids 407-419 of Human PCK2

  • 阳性对照
    • Human liver lysate and HepG2

性能

应用

Our Abpromise guarantee covers the use of ab40843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 71 kDa.

靶标

  • 功能
    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.
  • 通路
    Carbohydrate biosynthesis; gluconeogenesis.
  • 疾病相关
    Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD) [MIM:261650]. A metabolic disorder resulting from impaired gluconeogenesis. It is a rare disease with less than 10 cases reported in the literature. Clinical characteristics include hypotonia, hepatomegaly, failure to thrive, lactic acidosis and hypoglycemia. Autoposy reveals fatty infiltration of both the liver and kidneys. The disorder is transmitted as an autosomal recessive trait.
  • 序列相似性
    Belongs to the phosphoenolpyruvate carboxykinase [GTP] family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • EC 4.1.1.32 antibody
    • GTP mitochondrial precursor antibody
    • HGNC:8725 antibody
    • mitochondrial antibody
    • Mitochondrial phosphoenolpyruvate carboxykinase 2 antibody
    • OTTHUMP00000164700 antibody
    • PCK2 antibody
    • PCKGM_HUMAN antibody
    • PE antibody
    • PEP carboxykinase antibody
    • PEPCK antibody
    • PEPCK deficiency mitochondrial antibody
    • PEPCK M antibody
    • PEPCK-M antibody
    • PEPCK2 antibody
    • Phosphoenolpyruvate carboxykinase [GTP] antibody
    • Phosphoenolpyruvate carboxykinase 2 (mitochondrial) antibody
    • Phosphoenolpyruvate carboxykinase 2 mitochondrial antibody
    • Phosphoenolpyruvate carboxylase antibody
    • Phosphopyruvate carboxylase antibody
    see all

图片

  • Anti-PCK2 antibody (ab40843) at 2 µg/ml + Human Liver lysate at 35 µg/ml

    Predicted band size : 71 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)

    Primary incubation was 1 hour. Detected by chemiluminescence.

  • Anti-PCK2 antibody (ab40843) at 0.1 µg/ml + Human HepG2 lysate (35µg protein in RIPA buffer).

    Predicted band size : 71 kDa
    Observed band size : 71 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

实验方案

文献

This product has been referenced in:
  • Jamison RA  et al. Hyperglucagonemia precedes a decline in insulin secretion and causes hyperglycemia in chronically glucose-infused rats. Am J Physiol Endocrinol Metab 301:E1174-83 (2011). WB ; Rat . Read more (PubMed: 21862723) »
  • Wendel AA  et al. Glycerol-3-phosphate acyltransferase 1 deficiency in ob/ob mice diminishes hepatic steatosis but does not protect against insulin resistance or obesity. Diabetes 59:1321-9 (2010). WB ; Mouse . Read more (PubMed: 20200319) »

See all 4 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Chicken Cell lysate - other (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

提交于 Jan 11 2013

This statement is based on the total lack of overlap between the chosen immunizing peptide for PCK2 and any part of PCK1. Since the antibody is affinity purified using the immunizing peptide, it is reasonable to except that ab40843 will not cross react...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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