重组PE Anti-PBR抗体[EPR5384] (ab208836)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EPR5384] to PBR
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
概述
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产品名称
PE Anti-PBR抗体[EPR5384]
参阅全部 PBR 一抗 -
描述
PE兔单克隆抗体[EPR5384] to PBR -
宿主
Rabbit -
偶联物
PE. Ex: 488nm, Em: 575nm -
经测试应用
适用于: Flow Cyt (Intra), ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
(Peptide available asab170987) -
阳性对照
- Flow Cyt (intra): HeLa cells ICC/IF: HeLa cells
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5384 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-PBR antibody [EPR5384] (ab109497)
- Alexa Fluor® 488 Anti-PBR antibody [EPR5384] (ab199779)
- Alexa Fluor® 647 Anti-PBR antibody [EPR5384] (ab199836)
- Anti-PBR antibody [EPR5384] - BSA and Azide free (ab213654)
- Alexa Fluor® 594 Anti-PBR antibody [EPR5384] (ab303575)
- Alexa Fluor® 568 Anti-PBR antibody [EPR5384] (ab314401)
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab208836于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/500.
ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
1/100.
This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
说明 |
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Flow Cyt (Intra)
1/500. ab209478 - Rabbit monoclonal IgG (Phycoerythrin), is suitable for use as an isotype control with this antibody. |
ICC/IF
1/100. This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min) |
靶标
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功能
Responsible for the manifestation of peripheral-type benzodiazepine recognition sites and is most likely to comprise binding domains for benzodiazepines and isoquinoline carboxamides. May play a role in the transport of porphyrins and heme. Plays a role in the transport of cholesterol across mitochondrial membranes in steroidogenic cells. -
组织特异性
Found in many tissue types. Expressed at the highest levels under normal conditions in tissues that synthesize steroids. -
序列相似性
Belongs to the TspO/BZRP family. -
细胞定位
Mitochondrion membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 706 Human
- Entrez Gene: 12257 Mouse
- Omim: 109610 Human
- SwissProt: B1AH88 Human
- SwissProt: P30536 Human
- SwissProt: P50637 Mouse
- Unigene: 202 Human
- Unigene: 1508 Mouse
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别名
- Benzodiazapine receptor (peripheral) antibody
- Benzodiazepine peripheral binding site antibody
- BPBS antibody
see all
图片
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Overlay histogram showing HeLa cells stained with ab208836 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 90% methanol (-20°C) for 30 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208836, 1/500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycorythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 20 mW Solid State Blue Laser (488nm) and 585/40 bandpass filter.
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ab208836 staining PBR in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab208836 at 1/100 dilution (Pseudocolored in green) and ab195884, Rat monoclonal to Tubulin (Alexa Fluor® 647), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
实验方案
数据表及文件
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SDS download
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Datasheet download
Certificate of Compliance
文献 (1)
ab208836 被引用在 1 文献中.
- Loth MK et al. A Novel Interaction of Translocator Protein 18 kDa (TSPO) with NADPH Oxidase in Microglia. Mol Neurobiol 57:4467-4487 (2020). PubMed: 32743737