Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (NEURONAL)
Specification
NEURONAL
Fixative
Formaldehyde
Permeabilization
Yes - a. PBS + 0.2% Triton x-100
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/50
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS + 10% FBS+ 1%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit alexa 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/100
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提交于 Aug 30 2012