Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (E11.5 embryos)
Permeabilization
No
Specification
E11.5 embryos
Blocking step
PBSMBT (1X PBS, 1.5% Milk, 1.5% BSA, 0.1% Triton X-100) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBSMBT
Dilution
1/500
Secondary antibody
Dilution
1/250
Name
Non-Abcam antibody was used: Alexa Fluor® 568 Goat Anti-Rat IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 568
Additional data
Additional Notes
Embryos were fixed for 2hrs at 4°C in 2% PFA/PBS then placed in 30% sucrose O/N at 4°C prior to embedding in OCT using liquid nitrogen.
Blocking was O/N at 4°C.
1°Ab, 2°Ab and 3°Ab were all done for 1hr at RT.
Tertiary Ab. was Alexa Fluor 568 Donkey anti-Goat at 1/250 in PBSMBT at RT for 1hr.
Picture is assembled from multiple 10X images.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. William Wood
Verified customer
提交于 Apr 30 2014