Anti-PARVB抗体(ab80691)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to PARVB
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human PARVB aa 41-90 (N terminal).
    Sequence:

    LQEEGKNAINSPMSPALVDVHPEDTQLEENEERTMIDPTSKEDPKFKELV

  • 阳性对照
    • Transfected 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab80691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

图片

  • Anti-PARVB antibody (ab80691) at 1 µg/ml + transfected 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 42 kDa
    Observed band size: 45 kDa (why is the actual band size different from the predicted?)



    Gel concentration 12%

实验方案

文献

ab80691 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Xenopus laevis Tissue lysate - other (Whole Xenopus embryos)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
5 µg
Specification
Whole Xenopus embryos
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

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提交于 May 05 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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