Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (normal human fibroblasts)
Specification
normal human fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 1X PBS 0.25% Triton
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 37°C
Other product details
Dilution
1/500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: 1X PBS 1% BSA
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-mouse IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555
Dilution
1/500
Additional data
Additional Notes
The fixation step was performed in PFA 4% during 20 min at 4°C. The observed signal is good and the protein localization is as expected (cytoplasmic, nuclear and also probably mitochondrial). Then, this antibody seems to be specific and is suitable for immunofluorescence.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Paule Benit
Verified customer
提交于 Apr 23 2010