Application
Western blot
Sample
Zebrafish Tissue lysate - whole (Brain)
Loading amount
20 µg
Specification
Brain
Treatment
RIPA buffer, 4x Laemmli buffer
Gel Running Conditions
Reduced Denaturing (12% PAGE)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/5000
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%Milk in TBST
Secondary antibody
Name
Non-Abcam antibody was used: Amersham, NA934V HRP conjugated anti rabbit second
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: - kDa Non-specific: 30, 42 kDa
Positive control
Human Brain sample superior cortex - one specific band at around 26kDa
Additional data
Abcam response
We are sorry to hear that the antibody has not worked for Zebrafish (untested species).
An option would be to load as much sample as possible and use more concentrated antibody.
Longer exposure might show faint bands.
An option would be to load as much sample as possible and use more concentrated antibody.
Longer exposure might show faint bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Oct 27 2010