Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 10%)
Sample
Chinese Hamster Cell lysate - whole cell (CHO cells)
Specification
CHO cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 3% BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Perox-AffiniPure Dnk Anti-Rabbit IgG (H+L)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Positive control
the antibody was used in Hela cells and worked nicely
Additional data
Additional Notes
PVDF membrane, 1% Triton lysis buffer, protease inhibitor (EDTA-free) from Roche. Phosphatase inhibitor 2 and 3 (sigma)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 12 2014