PAI1 overexpression 293T裂解物(whole cell) (ab94070)
概述
-
产品名称
PAI1 overexpression 293T裂解物(whole cell)
参阅全部 PAI1 细胞裂解液 -
常规说明
ab94070 is a 293T cell transfected lysate in which Human PAI1 has been transiently over-expressed using a pCMV-PAI1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
-
经测试应用
适用于: WBmore details
性能
-
Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
研究领域
-
背景
Disease: Defects in SERPINE1 are the cause of plasminogen activator inhibitor-1 deficiency (PAI-1D) [MIM:613329]. It is a hematologic disorder characterized by increased bleeding after trauma, injury, or surgery. Affected females have menorrhagia. The bleeding defect is due to increased fibrinolysis of fibrin blood clots due to deficiency of plasminogen activator inhibitor-1, which inhibits tissue and urinary activators of plasminogen. Note=High concentrations of SERPINE1 seem to contribute to the development of venous but not arterial occlusions. Function: This inhibitor acts as 'bait' for tissue plasminogen activator, urokinase, and protein C. Its rapid interaction with TPA may function as a major control point in the regulation of fibrinolysis. PTM: Inactivated by proteolytic attack of the urokinase-type (u-PA) and the tissue-type (TPA), cleaving the 369-Arg- -Met-370 bond. Similarity: Belongs to the serpin family. Tissue specificity: Found in plasma and platelets and in endothelial, hepatoma and fibrosarcoma cells.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94070于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use at an assay dependent dilution.
|
说明 |
---|
WB
Use at an assay dependent dilution. |
图片
-
ab94070 at 15µg/lane on an SDS-PAGE gel.
-
All lanes : Anti-PAI1 antibody (ab89581) at 1/500 dilution
Lane 1 :PAI1 overexpression 293T lysate (whole cell) (ab94070)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
-
SDS download
-
Datasheet download
文献 (0)
ab94070 尚未被引用在任何文献中。