Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Dog Tissue lysate - whole (Brain)
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk in TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit IbG (HL) (DyLight680), polyclonal
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Host species: Goat
Clonality: Polyclonal
Conjugation: Other
Dilution
1/15000
Detection
Detection method
LI-COR Odyssey Scanner
Bands
Specific: 50 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
Adjacent bands may be a known cleavage product (at ~40kDa) and glycosylated form at ~55 kDa.
Infrared scanner intensity 5 (scan time is automatic the exposure time shown was entered to get the system to accept the review)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 06 2013