Anti-PADI4 / PAD4抗体(ab96758)
Key features and details
- Rabbit polyclonal to PADI4 / PAD4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-PADI4 / PAD4抗体
参阅全部 PADI4 / PAD4 一抗 -
描述
兔多克隆抗体to PADI4 / PAD4 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Recombinant fragment containing a sequence corresponding to a region within amino acids 326-581 of Human PADI4/ PAD4 (AAH25718).
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阳性对照
- WB: Transfected HEK-293 T whole cell lysate, human spleen tissue extract; IHC-P: Cal27 xenograft and mouse ovary tissue; IF: HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab96758于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
1/500 - 1/10000. Predicted molecular weight: 74 kDa.
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IHC-P | (1) |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/10000. Predicted molecular weight: 74 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Catalyzes the citrullination/deimination of arginine residues of proteins. Citrullinates histone H3 at 'Arg-8' and/or 'Arg-17' and histone H4 at 'Arg-3', which prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. -
组织特异性
Expressed in eosinophils and neutrophils, not expressed in peripheral monocytes or lymphocytes. -
疾病相关
Genetic variations in PADI4 are a cause of susceptibility to rheumatoid arthritis (RA) [MIM:180300]. It is a systemic inflammatory disease with autoimmune features and a complex genetic component. It primarily affects the joints and is characterized by inflammatory changes in the synovial membranes and articular structures, widespread fibrinoid degeneration of the collagen fibers in mesenchymal tissues, and by atrophy and rarefaction of bony structures. Note=Could have an important role in the pathogenesis of rheumatoid arthritis by increasing citrullination of proteins in rheumatoid arthritis synovial tissues, leading, in a cytokine-rich milieu, to a break in tolerance to citrullinated peptides processed and presented in the appropriate HLA context. -
序列相似性
Belongs to the protein arginine deiminase family. -
细胞定位
Cytoplasm. Nucleus. Cytoplasmic granule. Cytoplasmic granules of eosinophils and neutrophils. - Information by UniProt
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数据库链接
- Entrez Gene: 23569 Human
- Entrez Gene: 18602 Mouse
- Omim: 605347 Human
- SwissProt: Q9UM07 Human
- SwissProt: Q9Z183 Mouse
- Unigene: 522969 Human
- Unigene: 250358 Mouse
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别名
- EC 3.5.3.15 antibody
- HL 60 PAD antibody
- HL-60 PAD antibody
see all
图片
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All lanes : Anti-PADI4 / PAD4 antibody (ab96758) at 1/5000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : PAD4-transfected HEK-293T whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 74 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded Cal27 xenograft tissue staining PADI4/PAD4 with ab96758 at 1/100 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) whole cells
labeling PADI4/PAD4 with ab96758 at 1/200 dilution. -
Anti-PADI4 / PAD4 antibody (ab96758) at 1/1000 dilution + Human spleen tissue extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 74 kDa7.5% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded mouse ovary tissue staining PADI4/PAD4 at cytoplasm with ab96758 at 1/500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min.
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab96758 被引用在 6 文献中.
- Talal S et al. Neutrophil degranulation and severely impaired extracellular trap formation at the basis of susceptibility to infections of hemodialysis patients. BMC Med 20:364 (2022). PubMed: 36284314
- Ou Q et al. TcpC inhibits neutrophil extracellular trap formation by enhancing ubiquitination mediated degradation of peptidylarginine deiminase 4. Nat Commun 12:3481 (2021). PubMed: 34108482
- Ni Y et al. Interruption of neutrophil extracellular traps formation dictates host defense and tubular HOXA5 stability to augment efficacy of anti-Fn14 therapy against septic AKI. Theranostics 11:9431-9451 (2021). PubMed: 34646379
- Kosgodage US et al. Peptidylarginine Deiminase Inhibitors Reduce Bacterial Membrane Vesicle Release and Sensitize Bacteria to Antibiotic Treatment. Front Cell Infect Microbiol 9:227 (2019). PubMed: 31316918
- Liang Y et al. Inhibition of peptidylarginine deiminase alleviates LPS-induced pulmonary dysfunction and improves survival in a mouse model of lethal endotoxemia. Eur J Pharmacol 833:432-440 (2018). PubMed: 29981294
- Kolodziej S et al. PADI4 acts as a coactivator of Tal1 by counteracting repressive histone arginine methylation. Nat Commun 5:3995 (2014). ChIP, WB ; Human . PubMed: 24874575