Anti-Otx2抗体(ab76748)
Key features and details
- Rabbit polyclonal to Otx2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-Otx2抗体
参阅全部 Otx2 一抗 -
描述
兔多克隆抗体to Otx2 -
宿主
Rabbit -
特异性
Reacts specifically with 31 kDa Otx2 protein. -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Recombinant fragment
预测可用于: Rat -
免疫原
Synthetic peptide derived from the N terminal domain of human Otx2
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阳性对照
- This antibody gave a positive siganl in OTX2 Overexpression Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Whole serum -
Concentration information loading...
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纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab76748于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa).
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说明 |
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WB
1/100 - 1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 32 kDa). |
靶标
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功能
Probably plays a role in the development of the brain and the sense organs. Can bind to the BCD target sequence (BTS): 5'-TCTAATCCC-3'. -
组织特异性
Expressed in brain. -
疾病相关
Defects in OTX2 are the cause of microphthalmia syndromic type 5 (MCOPS5) [MIM:610125]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Up to 80% of cases of microphthalia occur in association with syndromes that include non-ocular abnormalities. MCOPS5 patients manifest unilateral or bilateral microphthalmia/clinical anophthalmia and variable additional features including coloboma, microcornea, cataract, retinal dystrophy, hypoplasia or agenesis of the optic nerve, agenesis of the corpus callosum, developmental delay, joint laxity, hypotonia, and seizures. -
序列相似性
Belongs to the paired homeobox family. Bicoid subfamily.
Contains 1 homeobox DNA-binding domain. -
发展阶段
Embryo. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 305858 Rat
- SwissProt: Q64201 Rat
- Unigene: 35222 Rat
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别名
- CPHD6 antibody
- Homeobox protein OTX2 antibody
- MCOPS 5 antibody
see all
图片
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All lanes : Anti-Otx2 antibody (ab76748) at 1 µg/ml
Lane 1 : OTX1 Recombinant Protein
at 0.1 µg
Lane 2 : OTX2 Overexpression Lysate at 20 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 32 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Exposure time: 8 minutes
Otx1 Recombinant Protein was used as a negative control. This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab76748 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
数据表及文件
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Datasheet download
文献 (2)
ab76748 被引用在 2 文献中.
- Moreno-Mármol T et al. Stretching of the retinal pigment epithelium contributes to zebrafish optic cup morphogenesis. Elife 10:N/A (2021). PubMed: 34545806
- Japp AS et al. High-resolution genomic analysis does not qualify atypical plexus papilloma as a separate entity among choroid plexus tumors. J Neuropathol Exp Neurol 74:110-20 (2015). PubMed: 25575132