Anti-Osteopontin抗体[53] (ab69498)
Key features and details
- Mouse monoclonal [53] to Osteopontin
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
概述
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产品名称
Anti-Osteopontin抗体[53]
参阅全部 Osteopontin 一抗 -
描述
小鼠单克隆抗体[53] to Osteopontin -
宿主
Mouse -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein corresponding to Human Osteopontin.
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阳性对照
- WB: HeLa, MCF-7, A431, HEK-293 and Caki-2 lysates. IHC-P: Human breast cancer tissue.
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常规说明
This product was changed from ascites to tissue culture supernatant on 2nd March 2020. Lot numbers higher than GR3233119 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
53 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69498于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
1/1000. Predicted molecular weight: 33 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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说明 |
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WB
1/1000. Predicted molecular weight: 33 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
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功能
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
组织特异性
Bone. Found in plasma. -
序列相似性
Belongs to the osteopontin family. -
翻译后修饰
Extensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
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别名
- BNSP antibody
- Bone sialoprotein 1 antibody
- Bone sialoprotein I antibody
see all
图片
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All lanes : Anti-Osteopontin antibody [53] (ab69498) at 1/1000 dilution
Lane 1 : HeLa lysate
Lane 2 : MCF-7 lysate
Lane 3 : A431 lysate
Lane 4 : HEK-293 lysate
Lane 5 : Caki-2 lysate
Predicted band size: 33 kDa -
Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained withab69498 at 10 µg/ml.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with ab69498 at 10 µg/ml.
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Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast cancer tissue stained with ab69498 at 10 µg/ml.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (34)
ab69498 被引用在 34 文献中.
- Liu Q et al. Osteopontin inhibits osteoarthritis progression via the OPN/CD44/PI3K signal axis. Genes Dis 9:128-139 (2022). PubMed: 35005113
- Wang C et al. Serum anti-SPP1 autoantibody as a potential novel biomarker in detection of esophageal squamous cell carcinoma. BMC Cancer 22:932 (2022). PubMed: 36038839
- Nilsson G et al. White matter injury but not germinal matrix hemorrhage induces elevated osteopontin expression in human preterm brains. Acta Neuropathol Commun 9:166 (2021). PubMed: 34654477
- Yang C et al. microRNA-143-3p regulates odontogenic differentiation of human dental pulp stem cells through regulation of the osteoprotegerin-RANK ligand pathway by targeting RANK. Exp Physiol 105:876-885 (2020). PubMed: 32052500
- Xin BC et al. Berberine Promotes Osteogenic Differentiation of Human Dental Pulp Stem Cells Through Activating EGFR-MAPK-Runx2 Pathways. Pathol Oncol Res 26:1677-1685 (2020). PubMed: 31598896