Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cultured Cells (Primary hippocampal neurons)
Permeabilization
Yes - 0,25% Triton in PBS
Specification
Primary hippocampal neurons
Blocking step
Donkey Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: 5% Donkey serum in PBS
Dilution
1/50
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-rabbit Cy3
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
Strong staining compared to control conditions (secondary antibody only). Signal quite diffuse though.
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提交于 Jan 14 2011