Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain (cortex))
Antigen retrieval step
None
Permeabilization
Yes - Triton during block and staining
Specification
mouse brain (cortex)
Blocking step
PBS, 10%Horse serum, 0,2%BSA, 0,3%Triton as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS, 1%Horse serum, 0,2%BSA, 0,3%Triton
Dilution
1/300
Secondary antibody
Dilution
1/400
Name
Non-Abcam antibody was used: goat anti rabbit AlexaFluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
PFA-fixed and agarose imbedded mouse brains. After cutting, the sections were stored 30µm thick free floating in PBS/NaAzide.
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提交于 Feb 08 2013