概述

  • 产品名称
  • 描述
    山羊多克隆抗体to Oct-1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Chicken, Cow, Pig, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Oct-1 aa 69-83 (N terminal) (Cysteine residue).
    Sequence:

    NVQSKSNEESGDSQQ


    Database link: P14859

  • 阳性对照
    • Nuclear lysates of cell line HeLa

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Protein G purified
  • 纯化说明
    Purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab106947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 76 kDa). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources.

靶标

  • 功能
    Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.
  • 组织特异性
    Ubiquitous. Isoform 2 is lymphocyte-specific.
  • 序列相似性
    Belongs to the POU transcription factor family. Class-2 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • 翻译后修饰
    Phosphorylated by PRKDC.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • FLJ42836 antibody
    • NF A1 antibody
    • NF-A1 antibody
    • Oct 1 antibody
    • Oct 1B antibody
    • Oct-1 antibody
    • OCT1 antibody
    • Octamer binding protein 1 antibody
    • Octamer binding transcription factor 1 antibody
    • Octamer-binding protein 1 antibody
    • Octamer-binding transcription factor 1 antibody
    • OTF 1 antibody
    • OTF-1 antibody
    • OTF1 antibody
    • OTTHUMP00000032348 antibody
    • OTTHUMP00000032350 antibody
    • OTTHUMP00000032351 antibody
    • PO21 antibody
    • PO2F1 antibody
    • PO2F1_HUMAN antibody
    • POU class 2 homeobox 1 antibody
    • POU domain class 2 transcription factor 1 antibody
    • POU domain, class 2, transcription factor 1 antibody
    • POU2F1 antibody
    see all

图片

  • Anti-Oct-1 antibody (ab106947) at 0.5 µg/ml + HeLa cell nuclear lysate (in RIPA buffer for 1 hour) at 35 µg
    Developed using the ECL technique

    Predicted band size : 76 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab106947 has not yet been referenced specifically in any publications.

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