Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (U2OS)
Specification
U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-0.1%tween20-1%BSA
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: goat-anti-rabbit-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: 57 kDa Non-specific: 25 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
siLuciferase
Positive control
siOTUB2
Additional data
Additional Notes
U2OS cells were transfected with GFP-OTUB2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Carlos le Sage
Verified customer
提交于 Oct 01 2013