Anti-ORC4L抗体(ab9641)
Key features and details
- Goat polyclonal to ORC4L
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-ORC4L抗体
参阅全部 ORC4L 一抗 -
描述
山羊多克隆抗体to ORC4L -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human
预测可用于: Cow, Dog -
免疫原
Synthetic peptide corresponding to Human ORC4L aa 400-500 (C terminal). (NP_001177810.1; NP_001177811.1)
Database link: NP_002543.2 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
1 hour primary incubation is recommended for this product. |
说明 |
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent, however specific DNA sequences that define origins of replication have not been identified so far. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. -
疾病相关
Defects in ORC4 are the cause of Meier-Gorlin syndrome type 2 (MGORS2) [MIM:613800]. MGORS2 is a syndrome characterized by bilateral microtia, aplasia/hypoplasia of the patellae, and severe intrauterine and postnatal growth retardation with short stature and poor weight gain. Additional clinical findings include anomalies of cranial sutures, microcephaly, apparently low-set and simple ears, microstomia, full lips, highly arched or cleft palate, micrognathia, genitourinary tract anomalies, and various skeletal anomalies. While almost all cases have primordial dwarfism with substantial prenatal and postnatal growth retardation, not all cases have microcephaly, and microtia and absent/hypoplastic patella are absent in some. Despite the presence of microcephaly, intellect is usually normal. -
序列相似性
Belongs to the ORC4 family. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 529245 Cow
- Entrez Gene: 476141 Dog
- Entrez Gene: 5000 Human
- Entrez Gene: 26428 Mouse
- Entrez Gene: 295596 Rat
- Omim: 603056 Human
- SwissProt: Q2YDI2 Cow
- SwissProt: O43929 Human
see all -
别名
- Origin recognition complex, subunit 4, S. cerevisiae, homolog of antibody
- FLJ46668 antibody
- HSORC4 antibody
see all
图片
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Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Mouse heart lysate at 35 µg
Predicted band size: 50 kDa -
Anti-ORC4L antibody (ab9641) at 0.5 µg/ml + Rat Heart lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 50 kDa
Observed band size: 50 kDa -
ab9641 (2µg/ml) staining of Jurkat lysate (RIPA buffer, 1.4E5 cells per lane). Detected by western blot using chemiluminescence.
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All lanes : Anti-ORC4L antibody (ab9641) at 1 µg/ml
Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
数据表及文件
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Datasheet download
文献 (11)
ab9641 被引用在 11 文献中.
- Hossain M et al. Multiple, short protein binding motifs in ORC1 and CDC6 control the initiation of DNA replication. Mol Cell 81:1951-1969.e6 (2021). PubMed: 33761311
- Cheung MH et al. Human NOC3 is essential for DNA replication licensing in human cells. Cell Cycle 18:605-620 (2019). PubMed: 30741601
- Hossain M & Stillman B Opposing roles for DNA replication initiator proteins ORC1 and CDC6 in control of Cyclin E gene transcription. Elife 5:N/A (2016). IP ; Human . PubMed: 27458800
- Zhang R et al. Targeting GLI by GANT61 involves mechanisms dependent on inhibition of both transcription and DNA licensing. Oncotarget 7:80190-80207 (2016). ICC/IF ; Human . PubMed: 27863397
- Sherstyuk VV et al. Mapping of Replication Origins in the X Inactivation Center of Vole Microtus levis Reveals Extended Replication Initiation Zone. PLoS One 10:e0128497 (2015). WB . PubMed: 26038842