Anti-OBFC1抗体(ab89250)
Key features and details
- Mouse polyclonal to OBFC1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-OBFC1抗体
参阅全部 OBFC1 一抗 -
描述
小鼠多克隆抗体to OBFC1 -
宿主
Mouse -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length protein within Human OBFC1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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阳性对照
- Human spleen tissue lysate, HeLa cell lysate and OBFC1 transfected 293T cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab89250于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa.
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说明 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 42 kDa. |
靶标
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功能
Component of the CST complex, a complex that binds to single-stranded DNA and is required to protect telomeres from DNA degradation. The CST complex binds single-stranded DNA with high affinity in a sequence-independent manner, while isolated subunits bind DNA with low affinity by themselves. In addition to telomere protection, the CST complex has probably a more general role in DNA metabolism at non-telomeric sites. -
序列相似性
Belongs to the STN1 family.
Contains 1 OB DNA-binding domain. -
细胞定位
Nucleus. Chromosome > telomere. - Information by UniProt
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数据库链接
- Entrez Gene: 79991 Human
- Omim: 613128 Human
- SwissProt: Q9H668 Human
- Unigene: 62314 Human
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别名
- AAF 44 antibody
- AAF44 antibody
- Alpha accessory factor 44 antibody
see all
图片
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Anti-OBFC1 antibody (ab89250) at 1 µg/ml + human spleen tissue lysate at 50 µg
Predicted band size: 42 kDa
Observed band size: 42 kDa -
Anti-OBFC1 antibody (ab89250) at 1 µg/ml + HeLa cell lysate at 50 µg
Predicted band size: 42 kDa
Observed band size: 42 kDa -
All lanes : Anti-OBFC1 antibody (ab89250) at 1 µg/ml
Lane 1 : OBFC1 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
数据表及文件
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Datasheet download
文献 (5)
ab89250 被引用在 5 文献中.
- Lyu X et al. Human CST complex protects stalled replication forks by directly blocking MRE11 degradation of nascent-strand DNA. EMBO J 40:e103654 (2021). PubMed: 33210317
- Chai W et al. The Intrinsically Disordered Region in the Human STN1 OB-Fold Domain Is Important for Protecting Genome Stability. Biology (Basel) 10:N/A (2021). PubMed: 34681076
- Zhang M et al. Mammalian CST averts replication failure by preventing G-quadruplex accumulation. Nucleic Acids Res 47:5243-5259 (2019). PubMed: 30976812
- Wang F et al. Human CST has independent functions during telomere duplex replication and C-strand fill-in. Cell Rep 2:1096-103 (2012). PubMed: 23142664
- Stewart JA et al. Human CST promotes telomere duplex replication and general replication restart after fork stalling. EMBO J 31:3537-49 (2012). PubMed: 22863775