Anti-Nup153抗体[QE5] (ab24700)
Key features and details
- Mouse monoclonal [QE5] to Nup153
- Suitable for: ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-Nup153抗体[QE5]
参阅全部 Nup153 一抗 -
描述
小鼠单克隆抗体[QE5] to Nup153 -
宿主
Mouse -
特异性
This antibody could also recognise other NPC polypeptides, p250 and p62, apart from Nup153.
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经测试应用
适用于: ICC/IFmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Full length protein. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: U2OS, HepG2, HeLa, Human and Mouse cells. ICC: NIH3T3 cells
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
QE5 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab24700于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (2) |
Use a concentration of 1 µg/ml.
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说明 |
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ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Possible DNA-binding subunit of the nuclear pore complex (NPC). The repeat-containing domain may be involved in anchoring components of the pore complex to the pore membrane. -
序列相似性
Contains 4 RanBP2-type zinc fingers. -
结构域
Contains F-X-F-G repeats. -
细胞定位
Nucleus > nuclear pore complex. Located to the terminal ring structure of the nucleoplasmic cage. - Information by UniProt
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数据库链接
- Entrez Gene: 9972 Human
- Entrez Gene: 218210 Mouse
- Omim: 603948 Human
- SwissProt: P49790 Human
- Unigene: 601591 Human
- Unigene: 718703 Human
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别名
- 153 kDa nucleoporin antibody
- HNUP153 antibody
- N153 antibody
see all
图片
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ab24700 staining Nup153 in HeLa cells. The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab24700 at 0.4 µg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at +4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 µg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 µg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
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ab24700 staining Nup153 in NIH3T3 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab24700 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Immunocytochemical analysis of U2OS cells, labeling Nup153 with ab24700. Cells were formaldehyde fixed, permeabilized with NP40, and blocked with 3% BSA for 1 hour at 21°C. Immunostaining with ab24700 diluted 1/100 for 12 hours at 4°C.
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Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 4% formaldehyde and permeabilized in 0.2% Triton x100 for 10 minutes at room temperature, then washed 3 times in PBS. The fixation and permeabilization was carried out in a single step. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).
A: HeLa cells, ab24700 used at 4µg/ml
B: 3T3 cells, ab24700 used at 2µg/ml
C: 3T3 cells, ab24700 used at 4µg/ml
D: 3T3 cells, ab24700 used at 6.6µg/ml -
Methanol fixed HeLa stained with ab24700. This antibody brilliantly highlights the nuclear membrane (green). The golgi is stained with Giantin (yellow).
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Human and mouse cells treated with different concentrations of ab24700. The cells were fixed in 100% methanol for 20 minutes at -20°C, then washed once in PBS. The image shows clear staining of the nuclear envalope (green). The DNA is stained with DAPI (blue).
A: HeLa cells, ab24700 used at 4µg/ml
B: 3T3 cells, ab24700 used at 2µg/ml
C: 3T3 cells, ab24700 used at 4µg/ml
D: 3T3 cells, ab24700 used at 6.6µg/ml
数据表及文件
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SDS download
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Datasheet download
文献 (57)
ab24700 被引用在 57 文献中.
- Weber M et al. MINSTED nanoscopy enters the Ångström localization range. Nat Biotechnol 41:569-576 (2023). PubMed: 36344840
- Wu YL et al. Maximum-likelihood model fitting for quantitative analysis of SMLM data. Nat Methods 20:139-148 (2023). PubMed: 36522500
- Lincoln R et al. A general design of caging-group-free photoactivatable fluorophores for live-cell nanoscopy. Nat Chem 14:1013-1020 (2022). PubMed: 35864152
- Shiimori M et al. Suv4-20h2 protects against influenza virus infection by suppression of chromatin loop formation. iScience 24:102660 (2021). PubMed: 34169237
- Rebensburg SV et al. Sec24C is an HIV-1 host dependency factor crucial for virus replication. Nat Microbiol 6:435-444 (2021). PubMed: 33649557