Anti-Nup107抗体(ab73290)
Key features and details
- Rabbit polyclonal to Nup107
- Suitable for: WB, ICC, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Nup107抗体
参阅全部 Nup107 一抗 -
描述
兔多克隆抗体to Nup107 -
宿主
Rabbit -
经测试应用
适用于: WB, ICC, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human Nup107 (internal sequence). The immunogen is located within amino acids 370 - 420 (20 amino acid synthetic peptide) of NUP107.
Database link: P57740 -
阳性对照
- A549 cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab73290于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa).
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ICC |
Use a concentration of 10 µg/ml.
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ICC/IF |
Use a concentration of 10 µg/ml.
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 95 kDa (predicted molecular weight: 106 kDa). |
ICC
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
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功能
Plays a role in the nuclear pore complex (NPC) assembly and/or maintenance. Required for the assembly of peripheral proteins into the NPC. May anchor NUP62 to the NPC. -
组织特异性
Ubiquitously expressed in fetal and adult tissues. -
疾病相关
Nephrotic syndrome 11 -
序列相似性
Belongs to the nucleoporin Nup84/Nup107 family. -
细胞定位
Nucleus membrane. Nucleus, nuclear pore complex. Chromosome, centromere, kinetochore. Located on both the cytoplasmic and nuclear sides of the NPC core structure (PubMed:11564755). During mitosis, localizes to the kinetochores (PubMed:11564755). Dissociates from the dissasembled NPC structure late during prophase of mitosis (PubMed:11564755). - Information by UniProt
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数据库链接
- Entrez Gene: 57122 Human
- Omim: 607617 Human
- SwissProt: P57740 Human
- Unigene: 524574 Human
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别名
- 107 kDa nucleoporin antibody
- NU107_HUMAN antibody
- Nuclear pore complex protein Nup107 antibody
see all
图片
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Immunofluorescence of NUP107 in A549 cells using ab73290 at 10 ug/ml.
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Lane 1 : Anti-Nup107 antibody (ab73290) at 1 µg/ml
Lane 2 : Anti-Nup107 antibody (ab73290) at 2 µg/ml
All lanes : A549 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 106 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Additional bands at: 194 kDa. We are unsure as to the identity of these extra bands. -
ab73290 at 10µg/ml staining Nup107 in A549 cells by Immunocytochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab73290 被引用在 5 文献中.
- Shi R et al. Nucleoporin 107 Promotes the Survival of Tumor Cells in Cervical Cancers. Gynecol Obstet Invest 85:41-52 (2020). PubMed: 31487736
- Ben-Yishay R et al. Imaging within single NPCs reveals NXF1's role in mRNA export on the cytoplasmic side of the pore. J Cell Biol 218:2962-2981 (2019). PubMed: 31375530
- Gasset-Rosa F et al. Cytoplasmic TDP-43 De-mixing Independent of Stress Granules Drives Inhibition of Nuclear Import, Loss of Nuclear TDP-43, and Cell Death. Neuron 102:339-357.e7 (2019). PubMed: 30853299
- Guan Y et al. Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity. J Cell Mol Med N/A:N/A (2018). PubMed: 30506890
- Kane M et al. Nuclear pore heterogeneity influences HIV-1 infection and the antiviral activity of MX2. Elife 7:N/A (2018). PubMed: 30084827