Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: rt°C
Other product details
Dilution
1/50
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. Stem cell markers have been observed in cycling columnar cells at the crypt base (Nature 449, 1003-1007; 2007). The cells (nuclei of the simple columnar epithelium of the upper mucosa) immunoreactive for Nucleostemin in this image (green arrowheads) anatomically correspond to the same cells expected to be Lgr5 positivite. A red arrowhead indicates nucleolar positivity in one of many cell nuclei in the Lamina propria of the mucosa (this area is not known for stem cell positivity).
Abcam response
Many thanks for this feedback on ab70346.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Feb 15 2010