Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
35 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (7% Tris-Acetat)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Mouse IgG - Fc (HRP)
Dilution
1/3000
Detection
Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 84 kDa
Additional data
Additional Notes
All lanes were HeLa extract (35µg) and used as loading control for another WB
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Lukas Rambousek
Verified customer
提交于 Dec 22 2010