Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (18% gel)
Sample
Human Cell lysate - whole cell (Breast)
Specification
Breast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.1% TBS-T
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Mouse Secondary
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL
Bands
Specific: 11 kDa
Exposure
1 minute(s) and 0 second(s)
Negative control
siRNA against Noxa.
Additional data
Additional Notes
This antibody worked really well for recognition of Noxa. Although the band is fainter at basal level, upon treatment with my drug, Adozelesin at 2nM for 24 hours, I was able to detect a strong, clean Noxa band.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jan 02 2014