Application
Western blot
Sample
Human Cell lysate - whole cell (ovarian cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
100 µg
Specification
ovarian cancer cell lines
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Santa Cruz
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 125 kDa
Positive control
HeLa cells transfected with Notch-1 expression vector
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 01 2013