Anti-Nogo A抗体(ab62024)
Key features and details
- Rabbit polyclonal to Nogo A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Nogo A抗体 -
描述
兔多克隆抗体to Nogo A -
宿主
Rabbit -
特异性
ab62024 is specific for Nogo A and Nogo E. -
经测试应用
适用于: ICC/IF, IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Raised against a 19 amino acid synthetic peptide from near the centre of Human Nogo A (GenBank accession no. NP_065393).
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阳性对照
- WB: Human brain tissue lysate. IHC-P: Mouse brain tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62024于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 2.5 µg/ml.
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).Can be blocked with Human Nogo A peptide (ab234893).
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 2.5 µg/ml. |
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).Can be blocked with Human Nogo A peptide (ab234893). |
靶标
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功能
Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. -
组织特异性
Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific. -
序列相似性
Contains 1 reticulon domain. -
结构域
Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor. -
细胞定位
Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. - Information by UniProt
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数据库链接
- Entrez Gene: 57142 Human
- Entrez Gene: 68585 Mouse
- Entrez Gene: 83765 Rat
- Omim: 604475 Human
- SwissProt: Q9NQC3 Human
- SwissProt: Q99P72 Mouse
- SwissProt: Q9JK11 Rat
- Unigene: 727710 Human
see all -
别名
- ASY antibody
- Foocen antibody
- Human NogoA antibody
see all
图片
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Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/ml
Lane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : anti-rabbit IgG
Predicted band size: 130 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa. We are unsure as to the identity of these extra bands. -
Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nogo A with Anti-Nogo A antibody (ab62024) at 5μg/ml.
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Immunohistochemical (Formalin-fixed paraffin embedded sections) analysis of mouse Nogo A in brain tissue using ab62024 at 2.5µg/ml.
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Immunohistochemical analysis of 4% paraformaldehydefixed Nogo A in mouse brain tissue using ab8416 at 20µg/ml.
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Immunohistochemical analysis of 4% paraformaldehyde-fixed rat brain tissue labelled Nogo A with ab62024 at 20µg/ml (green), followed by DAPI (blue) and phylloidin (red).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (20)
ab62024 被引用在 20 文献中.
- Peng K et al. A timeline of oligodendrocyte death and proliferation following experimental subarachnoid hemorrhage. CNS Neurosci Ther 28:842-850 (2022). PubMed: 35150055
- Feng XF et al. Magnetic Resonance Imaging Investigation of Neuroplasticity After Ischemic Stroke in Tetramethylpyrazine-Treated Rats. Front Pharmacol 13:851746 (2022). PubMed: 35559236
- Ullah HMA et al. Nogo-A Is Critical for Pro-Inflammatory Gene Regulation in Myocytes and Macrophages. Cells 10:N/A (2021). PubMed: 33572505
- Wang YY et al. Nogo-A aggravates oxidative damage in oligodendrocytes. Neural Regen Res 16:179-185 (2021). PubMed: 32788474
- Wang D et al. Combination of Electroacupuncture and Constraint-Induced Movement Therapy Enhances Functional Recovery After Ischemic Stroke in Rats. J Mol Neurosci N/A:N/A (2021). PubMed: 34101150