Application
Western blot
Sample
Rat Cell lysate - whole cell (H9C2 cell line)
Loading amount
150000 cells
Specification
H9C2 cell line
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS T 1/2 + Milk 5%
Secondary antibody
Name
Non-Abcam antibody was used: ThermoScientific
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
LiteAblot
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 35 kDa Non-specific: 50, 80 kDa
Positive control
Heart Human Tissue Lysate (ab29431)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Maria Angela D'Amico
Verified customer
提交于 Dec 16 2011