I used this kit to measure nitric oxide (nitrate and nitrite combined) in the plasma of adult male red-winged blackbirds (Agelaius phoeniceus) housed in an outdoor aviary. Preliminary data suggests that the assay requires a minimum of 15uL of plasma per well (ie, 30uL plasma to run in duplicate) to produce repeatable results. Thus far, we have found birds to have levels of NOx between 14-250 uM. It appears that birds with an active infection could have elevated NOx.
Preliminary data also suggests that error can be easily introduced when ready-ing the samples for the assay by deproteinating plasma with PCA. I suspect that this is particularly problematic for samples with absorbance reading less than 0.2, but we need more evidence to be certain.
I did notice that the instructions on the web site were not the same as in the booklet sent. In the booklet I received, the instructions said to use the plate reader after adding the second Griess reagent with no wait time listed. On the online book an incubation time was listed (10 min). There were also inconsistencies in the the list of reagents provided and what was actually provided (We got plates, which is great!). The plates were labeled with a store in -20C, even though they're just plastic plates that isn't necessary.
Technical support was very helpful in answering questions quickly whenever I called - was really happy with the support.
Preliminary data also suggests that error can be easily introduced when ready-ing the samples for the assay by deproteinating plasma with PCA. I suspect that this is particularly problematic for samples with absorbance reading less than 0.2, but we need more evidence to be certain.
I did notice that the instructions on the web site were not the same as in the booklet sent. In the booklet I received, the instructions said to use the plate reader after adding the second Griess reagent with no wait time listed. On the online book an incubation time was listed (10 min). There were also inconsistencies in the the list of reagents provided and what was actually provided (We got plates, which is great!). The plates were labeled with a store in -20C, even though they're just plastic plates that isn't necessary.
Technical support was very helpful in answering questions quickly whenever I called - was really happy with the support.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 01 2016