Anti-Niemann Pick C2抗体(ab64183)
Key features and details
- Rabbit polyclonal to Niemann Pick C2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Niemann Pick C2抗体
参阅全部 Niemann Pick C2 一抗 -
描述
兔多克隆抗体to Niemann Pick C2 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee, Monkey -
免疫原
Synthetic peptide corresponding to Human Niemann Pick C2 aa 1-100 conjugated to keyhole limpet haemocyanin.
(Peptide available asab93308) -
阳性对照
- This antibody gave a positive signal in human testis tissue lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab64183于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa).
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IHC-P |
Use a concentration of 1 µg/ml.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 16 kDa). |
IHC-P
Use a concentration of 1 µg/ml. |
靶标
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功能
May be involved in the regulation of the lipid composition of sperm membranes during the maturation in the epididymis. -
组织特异性
Epididymis. -
疾病相关
Defects in NPC2 are the cause of Niemann-Pick disease type C2 (NPDC2) [MIM:607625]. A lysosomal storage disorder that affects the viscera and the central nervous system. It is due to defective intracellular processing and transport of low-density lipoprotein derived cholesterol. It causes accumulation of cholesterol in lysosomes, with delayed induction of cholesterol homeostatic reactions. Niemann-Pick disease type C2 has a highly variable clinical phenotype. Clinical features include variable hepatosplenomegaly and severe progressive neurological dysfunction such as ataxia, dystonia and dementia. The age of onset can vary from infancy to late adulthood. -
序列相似性
Belongs to the NPC2 family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 450192 Chimpanzee
- Entrez Gene: 10577 Human
- Omim: 601015 Human
- SwissProt: P61917 Chimpanzee
- SwissProt: P61916 Human
- SwissProt: P61918 Monkey
- Unigene: 433222 Human
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别名
- EDDM1 antibody
- Epididymal protein 1 antibody
- Epididymal secretory protein antibody
see all
图片
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Anti-Niemann Pick C2 antibody (ab64183) at 1 µg/ml + Human testis tissue lysate - total protein (ab30257) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 16 kDa
Observed band size: 20 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
Human Epididymal secretory protein E1 precursor (Niemann pick C2) contains a number of potential glycosylation sites (SwissProt) which may explain the higher migrating band. -
IHC image of ab64183 staining in Human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64183, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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