Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (skin)
Specification
skin
Fixative
perfusion fixation
Permeabilization
Yes - Triton X 100
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/100
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Cy3 conjugated AffiniPure F(ab')2 Donkey anti Goat
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Abcam response
Thank you for your feedback. This antibody hasn't been tested in mouse and therefore may not be suitable for this species. We however welcome others to test this antibody in mouse. If you would like to do so, please contact our scientific support team at technical@abcam.com and we may be able to give you a testing discount.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jan 02 2013