Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 24°C
Sample
Snail (Helix pomatia) Tissue sections (brain)
Specification
brain
Permeabilization
Yes - TX
Fixative
Paraformaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 24°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: One-step polymer
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Image notes: A - labeled neurons and axons in the cerebral ganglion (brain) of the snail. B - labeled thin axon bundles in a tentacular muscle (it is made by wholemount IHC, every other data above are the same as for the brain sections).
The incubation with the blocking agent was done at 24¹C for 2 hours.
The incubation with the primary antibody was done at 24¹C for 24 hours.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 27 2013