Anti-Nicastrin抗体(ab3444)
Key features and details
- Rabbit polyclonal to Nicastrin
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Nicastrin抗体
参阅全部 Nicastrin 一抗 -
描述
兔多克隆抗体to Nicastrin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Nicastrin aa 650-750.
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阳性对照
- WB: T98G, RSC96, mouse neuronal cells, HeLa, IMR-32, NIH/3T3, L6, mouse brain, rat brain
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
Primary antibody说明
Amyloid beta peptide is the major constituent of amyloid plaques in the brains of individuals afflicted with Alzheimer’s disease. This peptide is generated from the beta-amyloid precursor protein (beta APP) in a two-step process. The first step involves cleavage of the extracellular, amino-terminal domain of beta APP. Protein cleavage is performed by an aspartyl protease termed beta-secretase (BACE). This enzyme is synthesized as a propeptide that must be modified to the mature and active form by the prohormone convertase, furin. Beta APP cleavage by the mature form of BACE results in the cellular secretion of a segment of beta APP and a membrane-bound remnant. This remnant is then processed by another protease termed gamma-secretase. Gamma-secretase cleaves an intra-membrane site in the carboxyl-terminal domain of beta APP, thus generating the amyloid beta peptide. Gamma-secretase is believed to be a multi-subunit complex containing presenilin-1 and 2 as central components. Found associated with the presenilins is the transmembrane glycoprotein nicastrin. Nicastrin has been found to bind to the carboxyl-terminus of beta APP and helps to modulate the production of the amyloid beta peptide. Also found in the neurofibrillary lesions associated with Alzheimer’s disease is a protein termed Tau. Tau is a neuronal microtubule-associated protein found predominantly on axons. Tau functions to promote tubulin polymerization and stabilize microtubules. Tau, in its hyperphosphorylated form, is the major constituent of paired helical filaments (PHF), which are the building block of neurofibrillary lesions found in brain tissue of Alzheimer’s diseased patients. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa).
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说明 |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 78 kDa). |
靶标
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功能
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). It probably represents a stabilizing cofactor required for the assembly of the gamma-secretase complex. -
组织特异性
Widely expressed. -
序列相似性
Belongs to the nicastrin family. -
细胞定位
Membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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数据库链接
- Entrez Gene: 23385 Human
- Entrez Gene: 59287 Mouse
- Entrez Gene: 289231 Rat
- Omim: 605254 Human
- SwissProt: Q92542 Human
- SwissProt: P57716 Mouse
- SwissProt: Q8CGU6 Rat
- Unigene: 517249 Human
see all -
别名
- Anterior pharynx defective 2 antibody
- APH 2 antibody
- APH2 antibody
see all
图片
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All lanes : Anti-Nicastrin antibody (ab3444) at 1 µg/ml
Lane 1 : HeLa whole cell extract
Lane 2 : IMR-32 whole cell extract (human neuroblastoma cell line)
Lane 3 : NIH/3T3 whole cell extract (mouse embryo fibroblast cell line)
Lane 4 : L6 whole cell extract (Rat skeletal muscle cell line)
Lane 5 : mouse brain
Lane 6 : rat brain
Lysates/proteins at 60 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa -
All lanes : Anti-Nicastrin antibody (ab3444) at 2 µg/ml
Lane 1 : T98G membrane extract (Human glioblastoma cell line)
Lane 2 : RSC96 membrane extract (spontaneously immortalized rat Schwann cell line )
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Developed using the ECL technique.
Predicted band size: 78 kDa -
Western blot detection of Nicastrin on mouse primary cultured neuronal cells using ab3444.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab3444 被引用在 4 文献中.
- Liu Y et al. Plasmalogen attenuates the development of hepatic steatosis and cognitive deficit through mechanism involving p75NTR inhibition. Redox Biol 43:102002 (2021). PubMed: 33984602
- Pintchovski SA et al. Evidence that enzyme processivity mediates differential Aß production by PS1 and PS2. Curr Alzheimer Res 10:4-10 (2013). WB . PubMed: 22950863
- Steuble M et al. Calsyntenin-1 shelters APP from proteolytic processing during anterograde axonal transport. Biol Open 1:761-74 (2012). PubMed: 23213470
- Massey LK et al. Ubiquilin regulates presenilin endoproteolysis and modulates gamma-secretase components, Pen-2 and nicastrin. Biochem J 391:513-25 (2005). PubMed: 15975090