Anti-Neutrophil抗体[7/4] (ab53457)
Key features and details
- Rat monoclonal [7/4] to Neutrophil
- Suitable for: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-Neutrophil抗体[7/4]
参阅全部 Neutrophil 一抗 -
描述
大鼠单克隆抗体[7/4] to Neutrophil -
宿主
Rat -
特异性
Rat anti mouse Ly-6B.2, clone 7/4 recognizes the Ly-6B.2 antigen in 129J; AKR; C57BL/6; C57BL/10; C58; DBA/2; NZB; NZW; SJL; MFI; Swiss (PO) strains. A2G; A/Sn; ASW; BALB/c; C3H/HEH: CBA.T6T6 are negative or demonstrate very weak reactivity. -
经测试应用
适用于: IHC-P, WB, IHC-Fr, ICC/IF, Flow Cytmore details -
种属反应性
与反应: Mouse -
免疫原
Tissue, cells or virus corresponding to Neutrophil. Cultured bone marrow cells
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表位
Recognizes the Ly-6B.2 antigen. -
阳性对照
- Flow cytometry: Mouse peripheral blood granulocytes.
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常规说明
Ly-6B.2 is a ~25-30 kDa GPI-anchored, heavily glycosylated protein expressed on neutrophils, inflammatory monocytes and some activated macrophages. High levels of expression are seen in bone marrow, spleen, lung and lymph nodes. N-glycanase treatment of thioglycollate elicited peritoneal neutrophil lysates lowers the apparent molecular weight of Ly-6B.2 to ~15 kDa.
ab53457 has been successfully used for the immunomagnetic depletion of neutrophils during the enrichment of primitive hematopoietic cells from bone marrow and the depletion of myeloid cells in vivo.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
7/4 -
骨髓瘤
Y3/Ag1.2.3 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53457于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
Use at an assay dependent concentration. PubMed: 25505239
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WB |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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说明 |
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IHC-P
Use at an assay dependent concentration. PubMed: 25505239 |
WB
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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靶标
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细胞定位
Cell membrane; Lipid-anchor, GPI-anchor -
数据库链接
- Entrez Gene: 110453 Mouse
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别名
- Ly-6B antibody
- Ly6b antibody
- Lymphocyte antigen 6B antibody
- Lymphocyte antigen 6B Short name=Ly-6B antibody
数据表及文件
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Datasheet download
文献 (34)
ab53457 被引用在 34 文献中.
- McClellan BL et al. Murine brain tumor microenvironment immunophenotyping using mass cytometry. STAR Protoc 3:101357 (2022). PubMed: 35634359
- Earnest JT et al. The mechanistic basis of protection by non-neutralizing anti-alphavirus antibodies. Cell Rep 35:108962 (2021). PubMed: 33826892
- Li Y et al. Nuclear envelope rupture and NET formation is driven by PKCa-mediated lamin B disassembly. EMBO Rep 21:e48779 (2020). PubMed: 32537912
- Zaidan M et al. Signaling pathways predisposing to chronic kidney disease progression. JCI Insight 5:N/A (2020). PubMed: 32376805
- Ji W et al. Majie cataplasm provides a shield against asthmatic punch from the neuroimmune system. Heliyon 6:e03896 (2020). PubMed: 32478182