Anti-Neutrophil Elastase抗体(ab68672)
Key features and details
- Rabbit polyclonal to Neutrophil Elastase
- Suitable for: IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neutrophil Elastase抗体
参阅全部 Neutrophil Elastase 一抗 -
描述
兔多克隆抗体to Neutrophil Elastase -
宿主
Rabbit -
特异性
We have had mixed results for use of this antibody in IHC-P using Mouse tissue samples. The abReview data below from external researchers reports good staining with this antibody in Mouse tissue samples using IHC-Fr. -
经测试应用
适用于: IHC-Pmore details
不适用于: WB -
种属反应性
与反应: Mouse, Human
预测可用于: Horse, Dog -
免疫原
Synthetic peptide corresponding to Human Neutrophil Elastase aa 1-100 (internal sequence) conjugated to keyhole limpet haemocyanin.
(Peptide available asab68671) -
阳性对照
- IHC-P: Human spleen and mouse spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab68672于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (8) |
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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IHC-P
1/100 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Modifies the functions of natural killer cells, monocytes and granulocytes. Inhibits C5a-dependent neutrophil enzyme release and chemotaxis. -
组织特异性
Bone marrow cells. -
疾病相关
Defects in ELANE are a cause of cyclic haematopoiesis (CH) [MIM:162800]; also known as cyclic neutropenia. CH is an autosomal dominant disease in which blood-cell production from the bone marrow oscillates with 21-day periodicity. Circulating neutrophils vary between almost normal numbers and zero. During intervals of neutropenia, affected individuals are at risk for opportunistic infection. Monocytes, platelets, lymphocytes and reticulocytes also cycle with the same frequency.
Defects in ELANE are the cause of neutropenia severe congenital autosomal dominant type 1 (SCN1) [MIM:202700]. SCN1 is a disorder of hematopoiesis characterized by a maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections. -
序列相似性
Belongs to the peptidase S1 family. Elastase subfamily.
Contains 1 peptidase S1 domain. - Information by UniProt
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数据库链接
- Entrez Gene: 1991 Human
- Entrez Gene: 50701 Mouse
- Omim: 130130 Human
- SwissProt: P08246 Human
- SwissProt: Q3UP87 Mouse
- Unigene: 99863 Human
- Unigene: 262194 Mouse
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别名
- Bone marrow serine protease antibody
- ELA2 antibody
- ELANE antibody
see all
图片
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IHC image of Neutrophil Elastase staining in a section of formalin-fixed paraffin-embedded normal mouse spleen performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab68672, 5ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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IHC image of Neutrophil Elastase staining in human spleen formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68672, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Immunohistochemistical detection of Neutrophil Elastase using antibody (ab68672) on formaldehyde-fixed paraffin-embedded human spleen sections. Antigen retrieval step: heat mediated in citric acid pH6 buffer. Permeabilization: No Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody dilution 1/2000; incubation time: 2 hours in TBS/BSA/azide. Secondary Antibody: anti Rabbit IgG conjugated to biotin (1/200).
数据表及文件
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SDS download
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Datasheet download
文献 (178)
ab68672 被引用在 178 文献中.
- Musiu C et al. Fatal cytokine release syndrome by an aberrant FLIP/STAT3 axis. Cell Death Differ 29:420-438 (2022). PubMed: 34518653
- Hawez A et al. c-Abl kinase regulates neutrophil extracellular trap formation and lung injury in abdominal sepsis. Lab Invest 102:263-271 (2022). PubMed: 34732849
- Huang S et al. Encountering and Wrestling: Neutrophils Recognize and Defensively Degrade Graphene Oxide. Adv Healthc Mater 11:e2102439 (2022). PubMed: 34859964
- Wang Y et al. Xuanfei Baidu Decoction reduces acute lung injury by regulating infiltration of neutrophils and macrophages via PD-1/IL17A pathway. Pharmacol Res 176:106083 (2022). PubMed: 35033647
- Yamazaki K et al. Increased Vascular Permeability Due to Spread and Invasion of Vibrio vulnificus in the Wound Infection Exacerbates Potentially Fatal Necrotizing Disease. Front Microbiol 13:849600 (2022). PubMed: 35350614