Neutrophil Elastase抑制剂Assay 试剂盒(ab118971)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 40 min
概述
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产品名称
Neutrophil Elastase抑制剂Assay 试剂盒
参阅全部 Neutrophil Elastase 试剂盒 -
检测方法
Fluorescent -
检测类型
Quantitative -
检测时间
0h 40m -
产品概述
In Abcam's Neutrophil Elastase Inhibitor Screening Kit, NE hydrolyzes a specific fluorescent substrate to release the fluorescent group, which can be detected at Ex/Em = 400/505 nm. In presence of a potent Neutrophil Elastase inhibitor, the hydrolyzation of substrate will be inhibited or stopped. The kit provides a rapid, simple, sensitive, and reliable test suitable as a high throughput screening assay of Neutrophil Elastase inhibitors. For comparison of the relative efficacy of test inhibitors, a control inhibitor, SPCK (Ki = 10 µM for human leukocyte elastase) is included.
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说明
This product is manufactured by BioVision, an Abcam company and was previously called K782 Neutrophil Elastase Inhibitor Screening Kit (Fluorometric). K782-100 is the same size as the 100 test size of ab118971.
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平台
Microplate reader
性能
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存放说明
Store at -20°C. Please refer to protocols. -
组件 100 tests Assay Buffer XL 1 x 25ml Elastase Inhibitor 1 x 100µl Elastase Enzyme 1 vial Substrate VI 1 x 0.2ml -
研究领域
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相关性
Neutrophil Elastase (NE) is an aggressive and cytotoxic 29 kDa serine protease stored mainly in the azurophil granules of neutrophil granulocytes. It plays a role in the degradation of a wide range of extracellular matrix proteins, including fibronectin, laminin, proteoglycans, collagens, and elastin. When the extracellular NE concentration exceeds the buffering capacity of endogenous inhibitors, it becomes implicated in the signs, symptoms and disease progression of inflammatory lung disorders via its role in the inflammatory process, mucus overproduction and lung tissue damage. -
别名
- Bone marrow serine protease
- ELA2
- ELANE
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图片
数据表及文件
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SDS download
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Datasheet download
文献 (13)
ab118971 被引用在 13 文献中.
- Bujak T et al. Flower Extracts as Multifunctional Dyes in the Cosmetics Industry. Molecules 27:N/A (2022). PubMed: 35164187
- Ziemlewska A et al. Evaluation of Cosmetic and Dermatological Properties of Kombucha-Fermented Berry Leaf Extracts Considered to Be By-Products. Molecules 27:N/A (2022). PubMed: 35408743
- Nowak A et al. Epilobium angustifolium L. Extracts as Valuable Ingredients in Cosmetic and Dermatological Products. Molecules 26:N/A (2021). PubMed: 34200200
- Zagórska-Dziok M et al. Positive Effect of Cannabis sativa L. Herb Extracts on Skin Cells and Assessment of Cannabinoid-Based Hydrogels Properties. Molecules 26:N/A (2021). PubMed: 33557174
- Bujak T et al. Antioxidant and Cytoprotective Properties of Plant Extract from Dry Flowers as Functional Dyes for Cosmetic Products. Molecules 26:N/A (2021). PubMed: 34068613