Anti-Neuroserpin抗体(ab33077)
Key features and details
- Rabbit polyclonal to Neuroserpin
- Suitable for: WB, ICC/IF, IHC-FoFr
- Reacts with: Mouse, Rat
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neuroserpin抗体
参阅全部 Neuroserpin 一抗 -
描述
兔多克隆抗体to Neuroserpin -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-FoFrmore details -
种属反应性
与反应: Mouse, Rat
预测可用于: Human -
免疫原
Synthetic peptide corresponding to Rat Neuroserpin aa 350 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab36619) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33077于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | ||
ICC/IF | ||
IHC-FoFr | (1) |
说明 |
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IHC-FoFr: Use at an assay dependent concentration.
WB: 1/250. Detects bands of approximately 46 and 55 kDa (predicted molecular weight: 46 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Serine protease inhibitor that inhibits plasminogen activators and plasmin but not thrombin. May be involved in the formation or reorganization of synaptic connections as well as for synaptic plasticity in the adult nervous system. May protect neurons from cell damage by tissue-type plasminogen activator. -
组织特异性
Predominantly expressed in the brain. -
疾病相关
Defects in SERPINI1 are the cause of familial encephalopathy with neuroserpin inclusion bodies (FEN1B) [MIM:604218]. FEN1B is characterized clinically as an autosomal dominantly inherited dementia, histologically by unique neuronal inclusion bodies and biochemically by polymers of neuroserpin. -
序列相似性
Belongs to the serpin family. -
细胞定位
Secreted. - Information by UniProt
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数据库链接
- Entrez Gene: 5274 Human
- Entrez Gene: 20713 Mouse
- Entrez Gene: 116459 Rat
- Omim: 602445 Human
- SwissProt: Q99574 Human
- SwissProt: O35684 Mouse
- SwissProt: Q9JLD2 Rat
- Unigene: 478153 Human
see all -
别名
- DKFZp781N13156 antibody
- Neuroserpin antibody
- NEUS_HUMAN antibody
see all
图片
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All lanes : Anti-Neuroserpin antibody (ab33077) at 1/250 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Neuroserpin contains a number of potential glycosylation sites so it is thought that this is the reason it runs at 55 kDa. The band size observed is also comparable to molecular weights seen with other commercially available antibodies to Neuroserpin. -
ICC/IF image of ab33077 stained rat PC12 cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab33077, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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All lanes : Anti-Neuroserpin antibody (ab33077) at 1 µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 46,55 kDa why is the actual band size different from the predicted?
Additional bands at: 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 10 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (6)
ab33077 被引用在 6 文献中.
- Higgins NR et al. Serpin neuropathology in the P497S UBQLN2 mouse model of ALS/FTD. Brain Pathol 31:e12948 (2021). PubMed: 33780087
- Adorjan I et al. Neuroserpin expression during human brain development and in adult brain revealed by immunohistochemistry and single cell RNA sequencing. J Anat N/A:N/A (2019). PubMed: 30644551
- Li W et al. Neuroprotective effect of neuroserpin in non-tPA-induced intracerebral hemorrhage mouse models. BMC Neurol 17:196 (2017). WB ; Mouse . PubMed: 29115923
- Kondo S et al. Secretory function in subplate neurons during cortical development. Front Neurosci 9:100 (2015). Mouse . PubMed: 25859180
- Roet KC et al. A multilevel screening strategy defines a molecular fingerprint of proregenerative olfactory ensheathing cells and identifies SCARB2, a protein that improves regenerative sprouting of injured sensory spinal axons. J Neurosci 33:11116-35 (2013). PubMed: 23825416
- Wu J et al. Neuroserpin protects neurons from ischemia-induced plasmin-mediated cell death independently of tissue-type plasminogen activator inhibition. Am J Pathol 177:2576-84 (2010). IHC-FoFr ; Mouse . PubMed: 20864675