Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (rat brain)
Specification
rat brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Other product details
Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti- rabbit Alexa fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
This antibody stained varicose fibers in the hypothalamus exclusively. The immunostaining was performed in free floating at the concentration of 1/1000 for the primary antobody (brain perfused with paraformaldehyde and cryoprotected). The pictures attached show at low power magnification the network of staining obtained and the varicose fibers at higher power magnification (X40).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Sophie Pezet
Verified customer
提交于 Dec 10 2007