Anti-Neuronatin抗体(ab27266)
Key features and details
- Rabbit polyclonal to Neuronatin
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neuronatin抗体
参阅全部 Neuronatin 一抗 -
描述
兔多克隆抗体to Neuronatin -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab27266于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa).
Abcam recommends using 1-3% Milk as the blocking agent. |
说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 9 kDa (predicted molecular weight: 9 kDa). Abcam recommends using 1-3% Milk as the blocking agent. |
靶标
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功能
May participate in the maintenance of segment identity in the hindbrain and pituitary development, and maturation or maintenance of the overall structure of the nervous system. May function as a regulatory subunit of ion channels. -
序列相似性
Belongs to the neuronatin family. -
发展阶段
Abundant in 18-24 week old fetal brain. Postnatally its expression decline and only minimal levels were present in adulthood. - Information by UniProt
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数据库链接
- Entrez Gene: 4826 Human
- Entrez Gene: 18111 Mouse
- Entrez Gene: 94270 Rat
- Omim: 603106 Human
- SwissProt: Q16517 Human
- SwissProt: Q61979 Mouse
- SwissProt: Q62649 Rat
- Unigene: 504703 Human
see all -
别名
- Neuronatin antibody
- NNAT antibody
- NNAT_HUMAN antibody
see all
图片
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Anti-Neuronatin antibody (ab27266) at 1 µg/ml +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 20 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa -
Anti-Neuronatin antibody (ab27266) at 1 µg/ml + Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (blocked with 3% Milk) at 50 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 9 kDa
Exposure time: 12 minutes -
Anti-Neuronatin antibody (ab27266) at 1 µg/ml +
Mouse brain tissue lysate - total protein (0 days) (ab7188) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 9 kDa
Observed band size: 10 kDa why is the actual band size different from the predicted?
Additional bands at: 70-75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (19)
ab27266 被引用在 19 文献中.
- Zou HY et al. Aberrant miR-339-5p/neuronatin signaling causes prodromal neuronal calcium dyshomeostasis in mutant presenilin mice. J Clin Invest 132:N/A (2022). PubMed: 35426376
- Sinha P et al. Trehalose Ameliorates Seizure Susceptibility in Lafora Disease Mouse Models by Suppressing Neuroinflammation and Endoplasmic Reticulum Stress. Mol Neurobiol 58:1088-1101 (2021). PubMed: 33094475
- Saeed H et al. Aberrant epigenetic silencing of neuronatin is a frequent event in human osteosarcoma. Oncotarget 11:1876-1893 (2020). PubMed: 32499872
- Kanno N et al. Characteristic Localization of Neuronatin in Rat Testis, Hair Follicle, Tongue, and Pancreas. J Histochem Cytochem 67:495-509 (2019). PubMed: 30869556
- Vatsa N et al. Down-Regulation of miRNA-708 Promotes Aberrant Calcium Signaling by Targeting Neuronatin in a Mouse Model of Angelman Syndrome. Front Mol Neurosci 12:35 (2019). PubMed: 30814928