Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 7% · Temperature: 25°C
Sample
Mouse Cell (DRG neurons)
Specification
DRG neurons
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS+3.5% Normal goat serum (NGS)
Dilution
1/10000
Secondary antibody
Name
Non-Abcam antibody was used: Alexa 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/1000
Additional data
Additional Notes
Please notice in the attached image, NF-positive cell is pointed by white arrow. Whole neuronal population are positive for TUJ1. As shown for the neuron pointed by red arrow, NF negative neuros are not stained, showing the antibody is highly specific in the examined range.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Shachar Dagan
Verified customer
提交于 Dec 16 2013