Anti-Neurofilament heavy polypeptide抗体(ab72996)
Key features and details
- Chicken polyclonal to Neurofilament heavy polypeptide
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgY
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Neurofilament heavy polypeptide抗体
参阅全部 Neurofilament heavy polypeptide 一抗 -
描述
鸡多克隆抗体to Neurofilament heavy polypeptide -
宿主
Chicken -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Rat -
免疫原
Full length native protein (purified) corresponding to Cow Neurofilament heavy polypeptide.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.065% Sodium azide
Constituent: Tissue culture supernatant -
Concentration information loading...
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纯度
IgY fraction -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72996于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa).
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说明 |
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WB
1/50000. Detects a band of approximately 200 kDa (predicted molecular weight: 115 kDa). |
靶标
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功能
Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. NF-H has an important function in mature axons that is not subserved by the two smaller NF proteins. -
疾病相关
Defects in NEFH are a cause of susceptibility to amyotrophic lateral sclerosis (ALS) [MIM:105400]. ALS is a neurodegenerative disorder affecting upper and lower motor neurons, and resulting in fatal paralysis. Sensory abnormalities are absent. Death usually occurs within 2 to 5 years. The etiology is likely to be multifactorial, involving both genetic and environmental factors. -
序列相似性
Belongs to the intermediate filament family. -
翻译后修饰
There are a number of repeats of the tripeptide K-S-P, NFH is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFH results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber.
Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincident with a change in the neurofilament function.
Phosphorylated in the Head and Rod regions by the PKC kinase PKN1, leading to inhibit polymerization. - Information by UniProt
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数据库链接
- Entrez Gene: 24587 Rat
- SwissProt: P16884 Rat
- Unigene: 108194 Rat
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别名
- 200 kDa neurofilament protein antibody
- CMT2CC antibody
- Nefh antibody
see all
图片
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Anti-Neurofilament heavy polypeptide antibody (ab72996) at 1/50000 dilution + Rat cerebral cortex lysate
Predicted band size: 115 kDa
Observed band size: 200 kDa why is the actual band size different from the predicted?
数据表及文件
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SDS download
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Datasheet download
文献 (33)
ab72996 被引用在 33 文献中.
- Guo Z et al. Engineering human skin model innervated with itch sensory neuron-like cells differentiated from induced pluripotent stem cells. Bioeng Transl Med 7:e10247 (2022). PubMed: 35111948
- Rose N et al. Receptor clustering and pathogenic complement activation in myasthenia gravis depend on synergy between antibodies with multiple subunit specificities. Acta Neuropathol 144:1005-1025 (2022). PubMed: 36074148
- Song DA et al. A New Monoclonal Antibody Enables BAR Analysis of Subcellular Importin β1 Interactomes. Mol Cell Proteomics 21:100418 (2022). PubMed: 36180036
- Iyer CC et al. Follistatin-induced muscle hypertrophy in aged mice improves neuromuscular junction innervation and function. Neurobiol Aging 104:32-41 (2021). PubMed: 33964607
- Otsu M et al. Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells. Front Cell Dev Biol 9:688283 (2021). PubMed: 34504841